Development and validation of a method for porphyrins quantification using HPLC-UV in urine
نویسندگان
چکیده
Porphyrias are the group of orphan diseases, related to pathological disruptions heme biosynthesis. These diseases hard diagnose timely and existing methods lack universality. High performance liquid chromatography coupled with ultraviolet detector could be adapted for this purpose via determination porphyrins in human urine. Waters Acquity UPLC H-CLASS diode matrix was used study. Developed method allows determining inner standard 9 biomarkers, each connected specific stage is validated porphyrias diagnosis.
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ژورنال
عنوان ژورنال: ??????????????? ? ???????????????
سال: 2022
ISSN: ['2587-7836', '2686-8830']
DOI: https://doi.org/10.37489/2587-7836-2022-2-36-45